Leveraging human genomic information to identify nonhuman primate sequences for expression array development.

ABSTRACT:

Background

Nonhuman primates (NHPs) are essential for biomedical research due to their similarities to humans. The utility of NHPs will be greatly increased by the application of genomics-based approaches such as gene expression profiling. Sequence information from the 3' end of genes is the key resource needed to create oligonucleotide expression arrays.

Results

We have developed the algorithms and procedures necessary to quickly acquire sequence information from the 3' end of nonhuman primate orthologs of human genes. To accomplish this, we identified terminal exons of over 15,000 human genes by aligning mRNA sequences with genomic sequence. We found the mean length of complete last exons to be approximately 1,400 bp, significantly longer than previous estimates. We design

SUBMITTER: Spindel ER 

PROVIDER: S-EPMC1314899 | biostudies-literature | 2005 Nov

REPOSITORIES: biostudies-literature