Characterization of 954 bovine full-CDS cDNA sequences.

ABSTRACT:

Background

Genome assemblies rely on the existence of transcript sequence to stitch together contigs, verify assembly of whole genome shotgun reads, and annotate genes. Functional genomics studies also rely on transcript sequence to create expression microarrays or interpret digital tag data produced by methods such as Serial Analysis of Gene Expression (SAGE). Transcript sequence can be predicted based on reconstruction from overlapping expressed sequence tags (EST) that are obtained by single-pass sequencing of random cDNA clones, but these reconstructions are prone to errors caused by alternative splice forms, transcripts from gene families with related sequences, and expressed pseudogenes. These errors confound genome assembly and annotation. The most useful transcript sequence

SUBMITTER: Harhay GP 

PROVIDER: S-EPMC1314900 | biostudies-literature | 2005 Nov

REPOSITORIES: biostudies-literature