Selection of reference genes for quantitative real-time PCR in bovine preimplantation embryos.

ABSTRACT:

Background

Real-time quantitative PCR is a sensitive and very efficient technique to examine gene transcription patterns in preimplantation embryos, in order to gain information about embryo development and to optimize assisted reproductive technologies. Critical to the successful application of real-time PCR is careful assay design, reaction optimization and validation to maximize sensitivity and accuracy. In most of the studies published GAPD, ACTB or 18S rRNA have been used as a single reference gene without prior verification of their expression stability. Normalization of the data using unstable controls can result in erroneous conclusions, especially when only one reference gene is used.

Results

In this study the transcription levels of 8 commonly used reference genes

SUBMITTER: Goossens K 

PROVIDER: S-EPMC1315359 | biostudies-literature | 2005 Dec

REPOSITORIES: biostudies-literature