A novel, inducible, eukaryotic gene expression system based on the quorum-sensing transcription factor TraR.

ABSTRACT: Bacteria adapt their pattern of gene expression in response to a variety of external cues, including fluctuations in population density. This type of bacterial cell-to-cell communication is referred to as quorum-sensing. Quorum-sensing systems are present in many bacterial species and constitute a large collection of ligands and cognate receptors. The availability of such diversity offers interesting opportunities for biotechnological exploitation. We describe here the transformation of the quorum-sensing system of Agrobacterium tumefaciens into a transcription regulatory system that works in mammalian cells. The A. tumefaciens TraR protein was fused to the eukaryotic activation domain of NF-kappaB p65, generating a novel chimaeric transcriptional activator that stimulates gene transcripti

SUBMITTER: Neddermann P 

PROVIDER: S-EPMC1315832 | biostudies-literature | 2003 Feb

REPOSITORIES: biostudies-literature