Simultaneous deficiency of both MurA and p60 proteins generates a rough phenotype in Listeria monocytogenes.

ABSTRACT: We examined eight spontaneously occurring rough mutants of Listeria monocytogenes for their ability to express two previously reported autolysins, p60 and MurA. All mutants lack MurA expression and show strongly reduced levels of extracellular p60. One rough strain harbors a variant of the p60 protein with a partially truncated catalytic domain. In seven cases there were shifts in the localization of p60 to the membrane fraction. Mutations within the secA2 gene, encoding an auxiliary protein secretion system paralog, were previously shown to be involved in the smooth-rough phenotypic variation seen with Listeria strains. An isogenic DeltasecA2 EGDe deletion strain displays a strong pleiotropic reduction of p60 and MurA, in addition to a large number of secreted and surface proteins. Howeve

SUBMITTER: Machata S 

PROVIDER: S-EPMC1317001 | biostudies-literature | 2005 Dec

REPOSITORIES: biostudies-literature