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DNA polymorphism and molecular subtyping of the capsular gene cluster of group B streptococcus.


ABSTRACT: Serotyping and other phenotypic methods are often used to characterize the capsular polysaccharide of group B streptococci (GBS). We describe a capsular genotyping method that utilizes PCR of capsular polysaccharide synthesis genes (cps) and restriction enzyme digestion. This method facilitates the detection of DNA polymorphism in cps genes and correlates well with serotyping.

SUBMITTER: Manning SD 

PROVIDER: S-EPMC1317180 | biostudies-literature | 2005 Dec

REPOSITORIES: biostudies-literature

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DNA polymorphism and molecular subtyping of the capsular gene cluster of group B streptococcus.

Manning Shannon D SD   Lacher David W DW   Davies H Dele HD   Foxman Betsy B   Whittam Thomas S TS  

Journal of clinical microbiology 20051201 12


Serotyping and other phenotypic methods are often used to characterize the capsular polysaccharide of group B streptococci (GBS). We describe a capsular genotyping method that utilizes PCR of capsular polysaccharide synthesis genes (cps) and restriction enzyme digestion. This method facilitates the detection of DNA polymorphism in cps genes and correlates well with serotyping. ...[more]

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