The role of 16S rRNA gene sequencing in identification of microorganisms misidentified by conventional methods.

ABSTRACT: Traditional methods for microbial identification require the recognition of differences in morphology, growth, enzymatic activity, and metabolism to define genera and species. Full and partial 16S rRNA gene sequencing methods have emerged as useful tools for identifying phenotypically aberrant microorganisms. We report on three bacterial blood isolates from three different College of American Pathologists-certified laboratories that were referred to ARUP Laboratories for definitive identification. Because phenotypic identification suggested unusual organisms not typically associated with the submitted clinical diagnosis, consultation with the Medical Director was sought and further testing was performed including partial 16S rRNA gene sequencing. All three patients had endocarditis, and co

SUBMITTER: Petti CA 

PROVIDER: S-EPMC1317215 | biostudies-literature | 2005 Dec

REPOSITORIES: biostudies-literature