Soluble lumican glycoprotein purified from human amniotic membrane promotes corneal epithelial wound healing.

ABSTRACT:

Purpose

To purify and characterize the glycoprotein lumican, isolated from human amniotic membrane (AM), and to examine its efficacy in treating corneal epithelium debridement.

Methods

An affinity-purified, anti-human lumican antibody-conjugated protein A Sepharose column was used to purify soluble lumican protein from human AM. The purified AM lumican was characterized by two-dimensional and SDS gel electrophoresis, plus Western blot analysis with anti-lumican antibody. The effects of lumican on corneal epithelial wound healing were examined in an organ culture mouse eye model.

Results

Lumican was found to be abundantly present in the stroma of human AM. It was extracted from the AM by isotonic, 1 M NaCl, and 4 M guanidine HCl solutions, suggesting that it is present

SUBMITTER: Yeh LK 

PROVIDER: S-EPMC1317307 | biostudies-literature | 2005 Feb

REPOSITORIES: biostudies-literature