Production and characterization of antibodies against each of the three subunits of the Bacillus cereus nonhemolytic enterotoxin complex.

ABSTRACT: The nonhemolytic enterotoxin (Nhe) is one of the two three-component enterotoxins which are responsible for diarrheal food poisoning syndrome caused by Bacillus cereus. To facilitate the detection of this toxin, consisting of the subunits NheA, NheB, and NheC, a complete set of high-affinity antibodies against each of the three components was established and characterized. A rabbit antiserum specific for the C-terminal part (15 amino acids) of NheC was produced using a respective synthetic peptide coupled to a protein carrier for immunization. Using purified B. cereus exoprotein preparations as immunogens, one monoclonal antibody against NheA and several antibodies against NheB were obtained. No cross-reactivity with other proteins produced by different strains of B. cereus was observed. A

SUBMITTER: Dietrich R 

PROVIDER: S-EPMC1317347 | biostudies-literature | 2005 Dec

REPOSITORIES: biostudies-literature