Characterization of a unique chromosomal copper resistance gene cluster from Xanthomonas campestris pv. vesicatoria.

ABSTRACT: We characterized the copper resistance genes in strain XvP26 of Xanthomonas campestris pv. vesicatoria, which was originally isolated from a pepper plant in Taiwan. The copper resistance genes were localized to a 7,652-bp region which, based on pulsed-field gel electrophoresis and Southern hybridization, was determined to be located on the chromosome. These genes hybridized only weakly, as determined by Southern analysis, to other copper resistance genes in Xanthomonas and Pseudomonas strains. We identified five open reading frames (ORFs) whose products exhibited high levels of amino acid sequence identity to the products of previously reported copper genes. Mutations in ORF1, ORF3, and ORF4 removed copper resistance, whereas mutations in ORF5 resulted in an intermediate copper resistance

SUBMITTER: Basim H 

PROVIDER: S-EPMC1317478 | biostudies-literature | 2005 Dec

REPOSITORIES: biostudies-literature