Sequencing of random Euplotes crassus macronuclear genes supports a high frequency of +1 translational frameshifting.

ABSTRACT: Programmed translational frameshifts have been identified in genes from a broad range of organisms, but typically only a very few genes in a given organism require a frameshift for expression. In contrast, a recent analysis of gene sequences available in GenBank from ciliates in the genus Euplotes indicated that >5% required one or more +1 translational frameshifts to produce their predicted protein products. However, this sample of genes was nonrandom, biased, and derived from multiple Euplotes species. To test whether there truly is an abundance of frameshift genes in Euplotes, and to more accurately assess their frequency, we sequenced a random sample of 25 cloned genes/macronuclear DNA molecules from Euplotes crassus. Three new candidate +1 frameshift genes were identified in the sampl

SUBMITTER: Klobutcher LA 

PROVIDER: S-EPMC1317503 | biostudies-literature | 2005 Dec

REPOSITORIES: biostudies-literature