A polycystin multiprotein complex constitutes a cholesterol-containing signalling microdomain in human kidney epithelia.

ABSTRACT: Polycystins are plasma membrane proteins that are expressed in kidney epithelial cells and associated with the progression of ADPKD (autosomal dominant polycystic kidney disease). A polycystin multiprotein complex, including adherens junction proteins, is thought to play an important role in cell polarity and differentiation. Sucrose gradient analyses and immunoprecipitation studies of primary human kidney epithelial cells showed the polycystins and their associated proteins E-cadherin and beta-catenin distributed in a complex with the raft marker flotillin-2, but not caveolin-1, in high-density gradient fractions. The integrity of the polycystin multiprotein complex was sensitive to cholesterol depletion, as shown by cyclodextrin treatment of immunoprecipitated complexes. The overexpresse

SUBMITTER: Roitbak T 

PROVIDER: S-EPMC1317661 | biostudies-literature | 2005 Nov

REPOSITORIES: biostudies-literature