Optical detection of rate-determining ion-modulated conformational changes of the ether-a-go-go K+ channel voltage sensor.

ABSTRACT: In voltage-dependent ether-à-go-go (eag) K+ channels, the process of activation is modulated by Mg2+ and other divalent cations, which bind to a site in the voltage sensor and slow channel opening. Previous analysis of eag ionic and gating currents indicated that Mg2+ has a much larger effect on ionic than gating current kinetics. From this, we hypothesized that ion binding modulates voltage sensor conformational changes that are poorly represented in gating current recordings. We have now tested this proposal by using a combined electrophysiological and optical approach. We find that a fluorescent probe attached near S4 in the voltage sensor reports on two phases of the activation process. One component of the optical signal corresponds to the main charge-moving conformational changes of

SUBMITTER: Bannister JP 

PROVIDER: S-EPMC1317915 | biostudies-literature | 2005 Dec

REPOSITORIES: biostudies-literature