Use of a mixed tissue RNA design for performance assessments on multiple microarray formats.

ABSTRACT: The comparability and reliability of data generated using microarray technology would be enhanced by use of a common set of standards that allow accuracy, reproducibility and dynamic range assessments on multiple formats. We designed and tested a complex biological reagent for performance measurements on three commercial oligonucleotide array formats that differ in probe design and signal measurement methodology. The reagent is a set of two mixtures with different proportions of RNA for each of four rat tissues (brain, liver, kidney and testes). The design provides four known ratio measurements of >200 reference probes, which were chosen for their tissue-selectivity, dynamic range coverage and alignment to the same exemplar transcript sequence across all three platforms. The data generated

SUBMITTER: Thompson KL 

PROVIDER: S-EPMC1322274 | biostudies-literature | 2005 Dec

REPOSITORIES: biostudies-literature