Essential genes of a minimal bacterium.
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ABSTRACT:
SUBMITTER: Glass JI
PROVIDER: S-EPMC1324956 | biostudies-literature | 2006 Jan
REPOSITORIES: biostudies-literature
Publications
Essential genes of a minimal bacterium.
Glass John I JI Assad-Garcia Nacyra N Alperovich Nina N Yooseph Shibu S Lewis Matthew R MR Maruf Mahir M Hutchison Clyde A CA Smith Hamilton O HO Venter J Craig JC
Proceedings of the National Academy of Sciences of the United States of America 20060103 2
Mycoplasma genitalium has the smallest genome of any organism that can be grown in pure culture. It has a minimal metabolism and little genomic redundancy. Consequently, its genome is expected to be a close approximation to the minimal set of genes needed to sustain bacterial life. Using global transposon mutagenesis, we isolated and characterized gene disruption mutants for 100 different nonessential protein-coding genes. None of the 43 RNA-coding genes were disrupted. Herein, we identify 382 o ...[more]
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