Carbohydrate microarray for profiling the antibodies interacting with Globo H tumor antigen.

ABSTRACT: Understanding the specificity of cell-surface carbohydrates interaction with antibodies and receptors is important for the development of new therapeutics and high-sensitivity diagnostics. This approach is, however, limited to the availability of natural and truncated sequences of the oligosaccharides and the sensitivity of the assay system. Reported here is the synthesis of the cancer antigen Globo H hexasaccharide, an epitope found on the cell surface of breast, prostate, and ovarian cancers, and its truncated sequences by using the programmable one-pot synthesis strategy. The saccharides were then arrayed covalently on glass slides with different density and used for the fluorencense-based binding analysis of two monoclonal antibodies against Globo H and the serum from breast cancer pat

SUBMITTER: Huang CY 

PROVIDER: S-EPMC1325001 | biostudies-literature | 2006 Jan

REPOSITORIES: biostudies-literature