Resolution of a structural competition involving dimeric G-quadruplex and its C-rich complementary strand.

ABSTRACT: The resolution of the dimeric intermolecular G-quadruplex/duplex competition of the telomeric DNA sequence 5'-TAG GGT TAG GGT-3' and of its complementary 5' ACC CTA ACC CTA-3' is reported. To achieve this goal, melting experiments of both sequences and of the mixtures of these sequences were monitored by molecular absorption, molecular fluorescence and circular dichroism spectroscopies. Molecular fluorescence measurements were carried out using molecular beacons technology, in which the 5'-TAG GGT TAG GGT-3' sequence was labelled with a fluorophore and a quencher at the ends of the strand. Mathematical analysis of experimental spectroscopic data was performed by means of multivariate curve resolution, allowing the calculation of concentration profiles and pure spectra of all resolved struc

SUBMITTER: Jaumot J 

PROVIDER: S-EPMC1325204 | biostudies-literature | 2006

REPOSITORIES: biostudies-literature