The peroxisomal multifunctional protein interacts with cortical microtubules in plant cells.

ABSTRACT:

Background

The plant peroxisomal multifunctional protein (MFP) possesses up to four enzymatic activities that are involved in catalyzing different reactions of fatty acid beta-oxidation in the peroxisome matrix. In addition to these peroxisomal activities, in vitro assays revealed that rice MFP possesses microtubule- and RNA-binding activities suggesting that this protein also has important functions in the cytosol.

Results

We demonstrate that MFP is an authentic microtubule-binding protein, as it localized to the cortical microtubule array in vivo, in addition to its expected targeting to the peroxisome matrix. MFP does not, however, interact with the three mitotic microtubule arrays. Microtubule co-sedimentation assays of truncated versions of MFP revealed that multiple mi

SUBMITTER: Chuong SD 

PROVIDER: S-EPMC1325227 | biostudies-literature | 2005 Nov

REPOSITORIES: biostudies-literature