Genome-wide identification of the regulatory targets of a transcription factor using biochemical characterization and computational genomic analysis.

ABSTRACT:

Background

A major challenge in computational genomics is the development of methodologies that allow accurate genome-wide prediction of the regulatory targets of a transcription factor. We present a method for target identification that combines experimental characterization of binding requirements with computational genomic analysis.

Results

Our method identified potential target genes of the transcription factor Ndt80, a key transcriptional regulator involved in yeast sporulation, using the combined information of binding affinity, positional distribution, and conservation of the binding sites across multiple species. We have also developed a mathematical approach to compute the false positive rate and the total number of targets in the genome based on the multiple select

SUBMITTER: Jolly ER 

PROVIDER: S-EPMC1326232 | biostudies-literature | 2005 Nov

REPOSITORIES: biostudies-literature