Linking enzyme sequence to function using Conserved Property Difference Locator to identify and annotate positions likely to control specific functionality.

ABSTRACT:

Background

Families of homologous enzymes evolved from common progenitors. The availability of multiple sequences representing each activity presents an opportunity for extracting information specifying the functionality of individual homologs. We present a straightforward method for the identification of residues likely to determine class specific functionality in which multiple sequence alignments are converted to an annotated graphical form by the Conserved Property Difference Locator (CPDL) program.

Results

Three test cases, each comprised of two groups of functionally-distinct homologs, are presented. Of the test cases, one is a membrane and two are soluble enzyme families. The desaturase/hydroxylase data was used to design and test the CPDL algorithm because a comparat

SUBMITTER: Mayer KM 

PROVIDER: S-EPMC1326233 | biostudies-literature | 2005 Nov

REPOSITORIES: biostudies-literature