Harshlight: a "corrective make-up" program for microarray chips.

ABSTRACT:

Background

Microscopists are familiar with many blemishes that fluorescence images can have due to dust and debris, glass flaws, uneven distribution of fluids or surface coatings, etc. Microarray scans do show similar artifacts, which might affect subsequent analysis. Although all but the starkest blemishes are hard to find by the unaided eye, particularly in high-density oligonucleotide arrays (HDONAs), few tools are available to help with the detection of those defects.

Results

We develop a novel tool, Harshlight, for the automatic detection and masking of blemishes in HDONA microarray chips. Harshlight uses a combination of statistic and image processing methods to identify three different types of defects: localized blemishes affecting a few probes, diffuse defects affec

SUBMITTER: Suarez-Farinas M 

PROVIDER: S-EPMC1327692 | biostudies-literature | 2005 Dec

REPOSITORIES: biostudies-literature