Apoptotic Signaling Across Breast Cancer Subtypes and Cryoablation-Induced Tissue Injury.

ABSTRACT: Apoptosis maintains tissue homeostasis, and its dysregulation is closely associated with breast cancer progression and therapeutic resistance. We performed an integrative analysis of apoptosis-related signaling in breast cancer tissues across five molecular subtypes and compared these patterns with systemic apoptotic responses following cryoablation of benign fibroadenomas. Gene expression profiling was conducted using mRNA microarrays and validated by qRT-PCR and ELISA. Apoptosis pathway activity was assessed with the MSigDB HALLMARK_APOPTOSIS gene set, including intrinsic and extrinsic pathway scoring and an apoptotic balance index (ABI). MicroRNA profiling combined with in silico analyses identified potential miRNA-mRNA interactions. A progressive shift toward reduced pro-apoptotic and

SUBMITTER: Panfil A 

PROVIDER: S-EPMC13299198 | biostudies-literature | 2026 Jun

REPOSITORIES: biostudies-literature