Cloning and porin activity of the major outer membrane protein P1 from Coxiella burnetii.

ABSTRACT: Coxiella burnetii, the etiological agent of Q fever, is a gram-negative obligate intracellular bacterium. Two striking characteristics of this microorganism are its ability to thrive within a phagolysosome and its ability to persist in the environment outside a host cell. These abilities have been attributed to the existence of C. burnetii developmental cycle variants: large-cell variants (LCV), small-cell variants (SCV), and small dense cells (SDC). Variants differ in protein profiles, including differential expression of a major outer membrane protein (MOMP) of C. burnetii, designated P1. The approximately 29-kDa MOMP is highly expressed in LCV, down-regulated in SCV, and not apparent in SDC. We sought to characterize P1 through purification of native protein for N-terminal analysis, clo

SUBMITTER: Varghees S 

PROVIDER: S-EPMC133000 | biostudies-literature | 2002 Dec

REPOSITORIES: biostudies-literature