Unusual intron conservation near tissue-regulated exons found by splicing microarrays.

ABSTRACT: Alternative splicing contributes to both gene regulation and protein diversity. To discover broad relationships between regulation of alternative splicing and sequence conservation, we applied a systems approach, using oligonucleotide microarrays designed to capture splicing information across the mouse genome. In a set of 22 adult tissues, we observe differential expression of RNA containing at least two alternative splice junctions for about 40% of the 6,216 alternative events we could detect. Statistical comparisons identify 171 cassette exons whose inclusion or skipping is different in brain relative to other tissues and another 28 exons whose splicing is different in muscle. A subset of these exons is associated with unusual blocks of intron sequence whose conservation in vertebrates

SUBMITTER: Sugnet CW 

PROVIDER: S-EPMC1331982 | biostudies-literature | 2006 Jan

REPOSITORIES: biostudies-literature