Transient expression vectors for functional genomics, quantification of promoter activity and RNA silencing in plants.

ABSTRACT:

Background

We describe novel plasmid vectors for transient gene expression using Agrobacterium, infiltrated into Nicotiana benthamiana leaves. We have generated a series of pGreenII cloning vectors that are ideally suited to transient gene expression, by removing elements of conventional binary vectors necessary for stable transformation such as transformation selection genes.

Results

We give an example of expression of heme-thiolate P450 to demonstrate effectiveness of this system. We have also designed vectors that take advantage of a dual luciferase assay system to analyse promoter sequences or post-transcriptional regulation of gene expression. We have demonstrated their utility by co-expression of putative transcription factors and the promoter sequence of potential tar

SUBMITTER: Hellens RP 

PROVIDER: S-EPMC1334188 | biostudies-literature | 2005 Dec

REPOSITORIES: biostudies-literature