Within the fold: assessing differential expression measures and reproducibility in microarray assays.

ABSTRACT:

Background

'Fold-change' cutoffs have been widely used in microarray assays to identify genes that are differentially expressed between query and reference samples. More accurate measures of differential expression and effective data-normalization strategies are required to identify high-confidence sets of genes with biologically meaningful changes in transcription. Further, the analysis of a large number of expression profiles is facilitated by a common reference sample, the construction of which must be carefully addressed.

Results

We carried out a series of 'self-self' hybridizations in which aliquots of the same RNA sample were labeled separately with Cy3 and Cy5 fluorescent dyes and co-hybridized to the same microarray. From this, we can analyze the intensity-dependent

SUBMITTER: Yang IV 

PROVIDER: S-EPMC133446 | biostudies-literature | 2002 Oct

REPOSITORIES: biostudies-literature