Identification of a chromosome-targeting domain in the human condensin subunit CNAP1/hCAP-D2/Eg7.

ABSTRACT: CNAP1 (hCAP-D2/Eg7) is an essential component of the human condensin complex required for mitotic chromosome condensation. This conserved complex contains a structural maintenance of chromosomes (SMC) family protein heterodimer and three non-SMC subunits. The mechanism underlying condensin targeting to mitotic chromosomes and the role played by the individual condensin components, particularly the non-SMC subunits, are not well understood. We report here characterization of the non-SMC condensin component CNAP1. CNAP1 contains two separate domains required for its stable incorporation into the complex. We found that the carboxyl terminus of CNAP1 possesses a mitotic chromosome-targeting domain that does not require the other condensin components. The same region also contains a functional

SUBMITTER: Ball AR 

PROVIDER: S-EPMC133980 | biostudies-literature | 2002 Aug

REPOSITORIES: biostudies-literature