Sub-plastidial localization of two different phage-type RNA polymerases in spinach chloroplasts.

ABSTRACT: Plant plastids contain a circular genome of approximately 150 kb organized into approximately 35 transcription units. The plastid genome is organized into nucleoids and attached to plastid membranes. This relatively small genome is transcribed by at least two different RNA polymerases, one being of the prokaryotic type and plastid-encoded (PEP), the other one being of the phage-type and nucleus-encoded (NEP). The presumed localization of a second phage-type RNA polymerase in plastids is still questionable. There is strong evidence for a sequential action of NEP and PEP enzymes during plant development attributing a prevailing role of NEP during early plant and plastid development, although NEP is present in mature chloroplasts. In the present paper, we have analysed two different NEP enzym

SUBMITTER: Azevedo J 

PROVIDER: S-EPMC1342036 | biostudies-literature | 2006

REPOSITORIES: biostudies-literature