Fingerprinting closely related xanthomonas pathovars with random nonamer oligonucleotide microarrays.

ABSTRACT: Current bacterial DNA-typing methods are typically based on gel-based fingerprinting methods. As such, they access a limited complement of genetic information and many independent restriction enzymes or probes are required to achieve statistical rigor and confidence in the resulting pattern of DNA fragments. Furthermore, statistical comparison of gel-based fingerprints is complex and nonstandardized. To overcome these limitations of gel-based microbial DNA fingerprinting, we developed a prototype, 47-probe microarray consisting of randomly selected nonamer oligonucleotides. Custom image analysis algorithms and statistical tools were developed to automatically extract fingerprint profiles from microarray images. The prototype array and new image analysis algorithms were used to analyze 14 c

SUBMITTER: Kingsley MT 

PROVIDER: S-EPMC134374 | biostudies-literature | 2002 Dec

REPOSITORIES: biostudies-literature