Human papilloma viruses and cervical tumours: mapping of integration sites and analysis of adjacent cellular sequences.

ABSTRACT:

Background

In cervical tumours the integration of human papilloma viruses (HPV) transcripts often results in the generation of transcripts that consist of hybrids of viral and cellular sequences. Mapping data using a variety of techniques has demonstrated that HPV integration occurred without obvious specificity into human genome. However, these techniques could not demonstrate whether integration resulted in the generation of transcripts encoding viral or viral-cellular sequences. The aim of this work was to map the integration sites of HPV DNA and to analyse the adjacent cellular sequences.

Methods

Amplification of the INTs was done by the APOT technique. The APOT products were sequenced according to standard protocols. The analysis of the sequences was performed using BLA

SUBMITTER: Klimov E 

PROVIDER: S-EPMC134465 | biostudies-literature | 2002 Oct

REPOSITORIES: biostudies-literature