Cis-Golgi matrix proteins move directly to endoplasmic reticulum exit sites by association with tubules.

ABSTRACT: The role of cis-medial Golgi matrix proteins in retrograde traffic is poorly understood. We have used imaging techniques to understand the relationship between the cis-medial Golgi matrix and transmembrane proteins during retrograde traffic in control and brefeldin A (BFA)-treated cells. All five of the cis-medial matrix proteins tested were associated with retrograde tubules within 2-3 min of initiation of tubule formation. Then, at later time points (3-10 min), transmembrane proteins are apparent in the same tubules. Strikingly, both the matrix proteins and the transmembrane proteins moved directly to endoplasmic reticulum (ER) exit sites labeled with p58 and Sec13, and there seemed to be a specific interaction between the ER exit sites and the tips or branch points of the tubules enrich

SUBMITTER: Mardones GA 

PROVIDER: S-EPMC1345687 | biostudies-literature | 2006 Jan

REPOSITORIES: biostudies-literature