Identification of Listeria monocytogenes genes contributing to intracellular replication by expression profiling and mutant screening.

ABSTRACT: A successful transition of Listeria monocytogenes from the extracellular to the intracellular environment requires a precise adaptation response to conditions encountered in the host milieu. Although many key steps in the intracellular lifestyle of this gram-positive pathogen are well characterized, our knowledge about the factors required for cytosolic proliferation is still rather limited. We used DNA microarray and real-time reverse transcriptase PCR analyses to investigate the transcriptional profile of intracellular L. monocytogenes following epithelial cell infection. Approximately 19% of the genes were differentially expressed by at least 1.6-fold relative to their level of transcription when grown in brain heart infusion medium, including genes encoding transporter proteins essenti

SUBMITTER: Joseph B 

PROVIDER: S-EPMC1347271 | biostudies-literature | 2006 Jan

REPOSITORIES: biostudies-literature