Electron transport in the pathway of acetate conversion to methane in the marine archaeon Methanosarcina acetivorans.

ABSTRACT: A liquid chromatography-hybrid linear ion trap-Fourier transform ion cyclotron resonance mass spectrometry approach was used to determine the differential abundance of proteins in acetate-grown cells compared to that of proteins in methanol-grown cells of the marine isolate Methanosarcina acetivorans metabolically labeled with 14N versus 15N. The 246 differentially abundant proteins in M. acetivorans were compared with the previously reported 240 differentially expressed genes of the freshwater isolate Methanosarcina mazei determined by transcriptional profiling of acetate-grown cells compared to methanol-grown cells. Profound differences were revealed for proteins involved in electron transport and energy conservation. Compared to methanol-grown cells, acetate-grown M. acetivorans synthes

SUBMITTER: Li Q 

PROVIDER: S-EPMC1347274 | biostudies-literature | 2006 Jan

REPOSITORIES: biostudies-literature