Exploratory multi-omics analysis suggests context-dependent immune responses to vitamin D₃ supplementation in PBMCs of multiple sclerosis patients.

ABSTRACT:

Introduction

Vitamin D deficiency is a recognized risk factor for multiple sclerosis (MS), yet the in vivo molecular mechanisms linking vitamin D3 supplementation to immune regulation remain incompletely understood.

Methods

We performed longitudinal RNA sequencing (RNA-seq) and assay for transposase-accessible chromatin using sequencing (ATAC-seq) profiling of peripheral blood mononuclear cells (PBMCs) from patients with relapsing-remitting MS who either initiated daily high-dose vitamin D3 supplementation or continued regular supplementation, alongside healthy individuals receiving monthly vitamin D3 bolus supplementation. Within-individual changes over the follow-up period were assessed and integrated with published THP-1-derived vitamin D receptor (VDR) binding dat

SUBMITTER: Tripathi T 

PROVIDER: S-EPMC13472867 | biostudies-literature | 2026

REPOSITORIES: biostudies-literature