Regulation of SpeB in Streptococcus pyogenes by pH and NaCl: a model for in vivo gene expression.

ABSTRACT: For a pathogen such as Streptococcus pyogenes, ecological success is determined by its ability to sense the environment and mount an appropriate adaptive transcriptional response. Thus, determining conditions for analyses of gene expression in vitro that are representative of the in vivo environment is critical for understanding the contributions of transcriptional response pathways to pathogenesis. In this study, we determined that the gene encoding the SpeB cysteine protease is up-regulated over the course of infection in a murine soft-tissue model. Conditions were identified, including growth phase, acidic pH, and an NaCl concentration of <0.1 M, that were required for expression of speB in vitro. Analysis of global expression profiles in response to these conditions in vitro identified

SUBMITTER: Loughman JA 

PROVIDER: S-EPMC1347310 | biostudies-literature | 2006 Jan

REPOSITORIES: biostudies-literature