Weighted simulations redefining quadratic calibration: democratisation of analytical uncertainty evaluation.

ABSTRACT: Instrumental methods of analysis are often calibrated across broad analyte level ranges. Nevertheless, instrumental responses over such extended ranges are frequently nonlinear and heteroscedastic. Accurately assessing these responses, particularly the uncertainty associated with quantifications derived from calibration curves under these conditions, is challenging. Although simpler models may appear attractive, they require more assumptions that can be difficult to verify, even when suitable software is available, which considerably limits their widespread application. This study presents a weighted simulation method that enables reliable and straightforward weighted regression of quadratic relationships between instrumental responses and calibrator concentrations, while also supporting t

SUBMITTER: Lourenco FR 

PROVIDER: S-EPMC13473300 | biostudies-literature | 2026 Sep

REPOSITORIES: biostudies-literature