Identification of virulence genes in a pathogenic strain of Pseudomonas aeruginosa by representational difference analysis.

ABSTRACT: Pseudomonas aeruginosa is an opportunistic pathogen that may cause severe infections in humans and other vertebrates. In addition, a human clinical isolate of P. aeruginosa, strain PA14, also causes disease in a variety of nonvertebrate hosts, including plants, Caenorhabditis elegans, and the greater wax moth, Galleria mellonella. This has led to the development of a multihost pathogenesis system in which plants, nematodes, and insects have been used as adjuncts to animal models for the identification of P. aeruginosa virulence factors. Another approach to identifying virulence genes in bacteria is to take advantage of the natural differences in pathogenicity between isolates of the same species and to use a subtractive hybridization technique to recover relevant genomic differences. The s

SUBMITTER: Choi JY 

PROVIDER: S-EPMC134824 | biostudies-literature | 2002 Feb

REPOSITORIES: biostudies-literature