RaiIR genes are part of a quorum-sensing network controlled by cinI and cinR in Rhizobium leguminosarum.

ABSTRACT: Analysis of N-acyl-L-homoserine lactones (AHLs) produced by Rhizobium leguminosarum bv. viciae indicated that there may be a network of quorum-sensing regulatory systems producing multiple AHLs in this species. Using a strain lacking a symbiosis plasmid, which carries some of the quorum-sensing genes, we isolated mutations in two genes (raiI and raiR) that are required for production of AHLs. The raiIR genes are located adjacent to dad genes (involved in D-alanine catabolism) on a large indigenous plasmid. RaiR is predicted to be a typical LuxR-type quorum-sensing regulator and is required for raiI expression. The raiR gene was expressed at a low level, possibly from a constitutive promoter, and its expression was increased under the influence of the upstream raiI promoter. Using gene fusi

SUBMITTER: Wisniewski-Dye F 

PROVIDER: S-EPMC134902 | biostudies-literature | 2002 Mar

REPOSITORIES: biostudies-literature