Complete genomic sequence of SfV, a serotype-converting temperate bacteriophage of Shigella flexneri.

ABSTRACT: Bacteriophage SfV is a temperate serotype-converting phage of Shigella flexneri. SfV encodes the factors involved in type V O-antigen modification, and the serotype conversion and integration-excision modules of the phage have been isolated and characterized. We now report on the complete sequence of the SfV genome (37,074 bp). A total of 53 open reading frames were predicted from the nucleotide sequence, and analysis of the corresponding proteins was used to construct a functional map. The general organization of the genes in the SfV genome is similar to that of bacteriophage lambda, and numerous features of the sequence are described. The superinfection immunity system of SfV includes a lambda-like repression system and a P4-like transcription termination mechanism. Sequence analysis als

SUBMITTER: Allison GE 

PROVIDER: S-EPMC134923 | biostudies-literature | 2002 Apr

REPOSITORIES: biostudies-literature