The monoclonal antibody SM5-1 recognizes a fibronectin variant which is widely expressed in melanoma.

ABSTRACT:

Background

Previously we have generated the monoclonal antibody SM5-1 by using a subtractive immunization protocol of human melanoma. This antibody exhibits a high sensitivity for primary melanomas of 99% (248/250 tested) and for metastatic melanoma of 96% (146/151 tested) in paraffin embedded sections. This reactivity is superior to the one obtained by HMB-45, anti-MelanA or anti-Tyrosinase and is comparable to anti-S100. However, as compared to anti-S100, the antibody SM5-1 is highly specific for melanocytic lesions since 40 different neoplasms were found to be negative for SM5-1 by immunohistochemistry. The antigen recognized by SM5-1 is unknown.

Methods

In order to characterize the antigen recognized by mAb SM5-1, a cDNA library was constructed from the metastatic human

SUBMITTER: Trefzer U 

PROVIDER: S-EPMC1351261 | biostudies-literature | 2006 Jan

REPOSITORIES: biostudies-literature