Development and evaluation of a real-time PCR assay targeting the type III secretion system of Burkholderia pseudomallei.

ABSTRACT: Here we report on the development of a discriminatory real-time assay for the rapid identification of Burkholderia pseudomallei isolates and the evaluation of this assay for sensitivity against related species and detection in spiked human blood samples. The assay targets a 115-base-pair region within orf2 of the B. pseudomallei type III secretion system gene cluster and distinguishes B. pseudomallei from other microbial species. Assay performance was evaluated with 224 geographically, temporally, and clinically diverse B. pseudomallei isolates from the Centers for Disease Control and Prevention strain collection. This represents the first real-time PCR for rapid and sensitive identification of B. pseudomallei that has been tested for cross-reactivity with 23 Burkholderia mallei, 5 Burkhol

SUBMITTER: Novak RT 

PROVIDER: S-EPMC1351940 | biostudies-literature | 2006 Jan

REPOSITORIES: biostudies-literature