Real-time fluorogenic reverse transcription-PCR assays for detection of bacteriophage MS2.

ABSTRACT: Bacteriophage MS2 is used in place of pathogenic viruses in a wide variety of studies that range from testing of compounds for disinfecting surfaces to studying environmental transport and fate of pathogenic viruses in groundwater. MS2 is also used as a pathogen simulant in the research, development, and testing (including open air tests) of methods, systems, and devices for the detection of pathogens in both the battlefield and homeland defense settings. PCR is often used as either an integral part of such detection systems or as a reference method to assess the sensitivity and specificity of microbial detection. To facilitate the detection of MS2 by PCR, we describe here a set of real-time fluorogenic reverse transcription-PCR assays. The sensitivity of the assays (performed with primer

SUBMITTER: O'Connell KP 

PROVIDER: S-EPMC1352182 | biostudies-literature | 2006 Jan

REPOSITORIES: biostudies-literature