Effect of inhibition of acetoclastic methanogenesis on growth of archaeal populations in an anoxic model environment.

ABSTRACT: Methyl fluoride is frequently used to specifically inhibit acetoclastic methanogenesis, thus allowing determination of the relative contribution of acetate versus H2/CO2 to total CH4 production in natural environments. However, the effect of the inhibitor on growth of the target archaeal population has not yet been studied. Therefore, we incubated rice roots as an environmental model system under anoxic conditions in the presence and absence of CH3F, measured the activity and Gibbs free energy (DeltaG) of CH4 production, and determined the abundance of individual archaeal populations by using a combination of quantitative (real-time) PCR and analysis of terminal restriction fragment length polymorphism targeting the 16S rRNA gene. It was shown that CH3F specifically inhibited not only acet

SUBMITTER: Penning H 

PROVIDER: S-EPMC1352266 | biostudies-literature | 2006 Jan

REPOSITORIES: biostudies-literature