Developmental regulation of the Streptomyces lividans ram genes: involvement of RamR in regulation of the ramCSAB operon.

ABSTRACT: Streptomycetes are filamentous soil bacteria that produce spores through a complex process of morphological differentiation. The ram cluster plays an important part during the development. The ram genes encode a membrane-bound kinase (RamC), a small protein (RamS), components of an ABC transporter (RamAB), and a response regulator (RamR). While the introduction of an extra copy of the ram cluster accelerates development in Streptomyces lividans, ramABR disruption mutants are unable to produce aerial hyphae and spores. The developmental regulation of ram gene transcription was analyzed. Transcription of the ram genes occurred only on solid rich media and not on minimal media. The ramR gene is transcribed from a single promoter during all growth stages, with the highest levels during aerial

SUBMITTER: Keijser BJ 

PROVIDER: S-EPMC135246 | biostudies-literature | 2002 Aug

REPOSITORIES: biostudies-literature