Comparative proteome analysis of Brucella melitensis vaccine strain Rev 1 and a virulent strain, 16M.

ABSTRACT: The genus Brucella consists of bacterial pathogens that cause brucellosis, a major zoonotic disease characterized by undulant fever and neurological disorders in humans. Among the different Brucella species, Brucella melitensis is considered the most virulent. Despite successful use in animals, the vaccine strains remain infectious for humans. To understand the mechanism of virulence in B. melitensis, the proteome of vaccine strain Rev 1 was analyzed by two-dimensional gel electrophoresis and compared to that of virulent strain 16M. The two strains were grown under identical laboratory conditions. Computer-assisted analysis of the two B. melitensis proteomes revealed proteins expressed in either 16M or Rev 1, as well as up- or down-regulation of proteins specific for each of these strains.

SUBMITTER: Eschenbrenner M 

PROVIDER: S-EPMC135307 | biostudies-literature | 2002 Sep

REPOSITORIES: biostudies-literature