Experimental validation of the regulated expression of large numbers of non-coding RNAs from the mouse genome.

ABSTRACT: Recent large-scale analyses of mainly full-length cDNA libraries generated from a variety of mouse tissues indicated that almost half of all representative cloned sequences did not contain an apparent protein-coding sequence, and were putatively derived from non-protein-coding RNA (ncRNA) genes. However, many of these clones were singletons and the majority were unspliced, raising the possibility that they may be derived from genomic DNA or unprocessed pre-mRNA contamination during library construction, or alternatively represent nonspecific "transcriptional noise." Here we show, using reverse transcriptase-dependent PCR, microarray, and Northern blot analyses, that many of these clones were derived from genuine transcripts of unknown function whose expression appears to be regulated. The

SUBMITTER: Ravasi T 

PROVIDER: S-EPMC1356124 | biostudies-literature | 2006 Jan

REPOSITORIES: biostudies-literature