Therapeutic protein transduction of mammalian cells and mice by nucleic acid-free lentiviral nanoparticles.

ABSTRACT: The straightforward production and dose-controlled administration of protein therapeutics remain major challenges for the biopharmaceutical manufacturing and gene therapy communities. Transgenes linked to HIV-1-derived vpr and pol-based protease cleavage (PC) sequences were co-produced as chimeric fusion proteins in a lentivirus production setting, encapsidated and processed to fusion peptide-free native protein in pseudotyped lentivirions for intracellular delivery and therapeutic action in target cells. Devoid of viral genome sequences, protein-transducing nanoparticles (PTNs) enabled transient and dose-dependent delivery of therapeutic proteins at functional quantities into a variety of mammalian cells in the absence of host chromosome modifications. PTNs delivering Manihot esculenta li

SUBMITTER: Link N 

PROVIDER: S-EPMC1356536 | biostudies-literature | 2006 Jan

REPOSITORIES: biostudies-literature