Stable and efficient cassette exchange under non-selectable conditions by combined use of two site-specific recombinases.

ABSTRACT: Work of the last decade has proven the 'one gene- one product-one function' hypothesis an oversimplification. To further unravel the emerging 'one gene-multiple products-even more functions' concept, new methods (such as subtle knock-in and tightly regulated conditional mutations) for the analysis of gene function in health and disease are required. Another class of improvements (such as tetraploid fusion and cassette exchange) addresses the efficiency with which targeted mutant strains can be generated. Recombinase-mediated cassette exchange (RMCE), which in theory is well suited for the rapid generation of multiple alleles of a given locus, is hampered by its low efficiency in the absence of selection and, especially in vivo, by the promiscuity of the participating recombinase recognitio

SUBMITTER: Lauth M 

PROVIDER: S-EPMC135837 | biostudies-literature | 2002 Nov

REPOSITORIES: biostudies-literature