Designing gene libraries from protein profiles for combinatorial protein experiments.

ABSTRACT: Protein combinatorial libraries provide new ways to probe the determinants of folding and to discover novel proteins. Such libraries are often constructed by expressing an ensemble of partially random gene sequences. Given the intractably large number of possible sequences, some limitation on diversity must be imposed. A non-uniform distribution of nucleotides can be used to reduce the number of possible sequences and encode peptide sequences having a predetermined set of amino acid probabilities at each residue position, i.e., the amino acid sequence profile. Such profiles can be determined by inspection, multiple sequence alignment or physically-based computational methods. Here we present a computational method that takes as input a desired sequence profile and calculates the individual

SUBMITTER: Wang W 

PROVIDER: S-EPMC135844 | biostudies-literature | 2002 Nov

REPOSITORIES: biostudies-literature