A Role for the SmpB-SsrA system in Yersinia pseudotuberculosis pathogenesis.

ABSTRACT: Yersinia utilizes a sophisticated type III secretion system to enhance its chances of survival and to overcome the host immune system. SmpB (small protein B) and SsrA (small stable RNA A) are components of a unique bacterial translational control system that help maintain the bacterial translational machinery in a fully operational state. We have found that loss of the SmpB-SsrA function causes acute defects in the ability of Yersinia pseudotuberculosis to survive in hostile environments. Most significantly, we show that mutations in smpB-ssrA genes render the bacterium avirulent and unable to cause mortality in mice. Consistent with these observations, we show that the mutant strain is unable to proliferate in macrophages and exhibits delayed Yop-mediated host cell cytotoxicity. Correspon

SUBMITTER: Okan NA 

PROVIDER: S-EPMC1358943 | biostudies-literature | 2006 Jan

REPOSITORIES: biostudies-literature